Yazar "Karaoğlu, Hakan" için listeleme
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The ATPase activity of the G2alt gene encoding an aluminium tolerance protein from Anoxybacillus gonensis G2
Beriş, Fatih Şaban; De Smet, Lina; Karaoğlu, Hakan; Çanakcı, Sabriye; Van Beeumen, Jozef; Belduz, Ali Osman (Springer, 2011)The G2ALT gene was cloned and sequenced from the thermophilic bacterium Anoxybacillus gonensis G2. The gene is 666 bp long and encodes a protein 221 amino acids in length. The gene was overexpressed in E. coli and purified ... -
Biochemical characterization of a novel glucose isomerase from Anoxybacillus gonensis G2(T) that displays a high level of activity and thermal stability
Karaoğlu, Hakan; Yanmış, Derya; Sal, Fulya Ay; Çelik, Ayhan; Çanakcı, Sabriye; Belduz, Ali Osman (Elsevier Science Bv, 2013)In the continuing search for novel enzymes suitable for the production of high fructose corn syrup (HFCS), a new glucose isomerase (GI) from the thermophile Anoxybacillus gonensis G2(T) is described. the gene encoding this ... -
Characterization of a highly thermostable recombinant xylanase from Anoxybacillus ayderensis
Akpınar, Züleyha; Karaoğlu, Hakan (Elsevier, 2024)Xylanases are the main enzymes to hydrolyze xylan, the major hemicellulose found in lignocellulose. Xylanases also have a wide range of industrial applications. Therefore, the discovery of new xylanases has the potential ... -
Characterization of a novel xylose isomerase from Anoxybacillus gonensis G2(T)
Yanmış, Derya; Karaoğlu, Hakan; Çolak, Dilşat Nigar; Sal, Fulya Ay; Çanakcı, Sabriye; Belduz, Ali Osman (Tubitak Scientific & Technical Research Council Turkey, 2014)The xylA gene encoding xylose isomerase from Anoxybacillus gonensis G2(T) has been cloned and successfully expressed in E. coli. Xylose isomerase was purified 10.98-fold by heat-shock and sequential column chromatography ... -
Characterization of a novel xylose isomerase from Anoxybacillus gonensis G2T
Yanmış, Derya; Karaoğlu, Hakan; Çolak, Dilşat Nigar; Şal, Fulya Ay; Çanakçı, Sabriye; Beldüz, Ali Osman (2014)The xylA gene encoding xylose isomerase from Anoxybacillus gonensis G2T has been cloned and successfully expressed in E. coli. Xylose isomerase was purified 10.98-fold by heat-shock and sequential column chromatography ... -
Cloning and determination of kinetic activity of thermophilic GCH-I from anoxybacillus flavithermus DSM 2641T
Phenylketonuria (PKU) is a disease caused by phenylalanine hydroxylase enzyme deficiency in newborn infants and is the most important cause of treatable mental retardation. One of the causes of the disease comes from the ... -
Cloning, expression, and characterization of a novel CTP synthase gene from Anoxybacillus gonensis G2
Sandallı, Cemal; Saral, Ayşegül; Ülker, Serdar; Karaoğlu, Hakan; Beldüz, Ali Osman; Çiçek, Ayşegül Çopur (2014)The cytidine-5'-triphosphate (CTP) synthase (EC 6.4.3.2) gene (pyrG) was cloned and sequenced from the thermophilic bacterium Anoxybacillus gonensis G2 (Ago). The gene is 1590 bp in length and encodes a protein of 530 amino ... -
Cloning, expression, purification and characterization of fructose-1,6-bisphosphate aldolase from Anoxybacillus gonensis G2
Ertunga, Nagihan Sağlam; Çolak, Ahmet; Belduz, Ali Osman; Çanakcı, Sabriye; Karaoğlu, Hakan; Sandallı, Cemal (2007)The fructose-1,6-bisphosphate aldolase gene from the thermophilic bacterium, Anoxybacillus gonensis G2, was cloned and sequenced. Nucleotide sequence analysis revealed an open reading frame coding for a 30.9 kDa protein ... -
Cloning, purification and characterization of a thermostable carboxylesterase from Anoxybacillus sp. PDF1
Ay, Fulya; Karaoğlu, Hakan; İnan, Kadriye; Çanakcı, Sabriye; Belduz, Ali Osman (Elsevier, 2011)The gene encoding a carboxylesterase from Anoxybacillus sp., PDF1, was cloned and sequenced. The recombinant protein was expressed in Escherichia coli BL21, under the control of isopropyl-?-d-thiogalactopyranoside-inducible ... -
Distribution of beta-lactamase genes among carbapenem-resistant klebsiella pneumoniae strains isolated from patients in Turkey
Iraz, Meryem; Düzgün, Azer Özad; Sandallı, Cemal; Doymaz, Mehmet Ziya; Akkoyunlu, Yasemin; Saral, Ayşegül; Peleg, Anton Y.; Özgümüş, Osman Birol; Beriş, Fatih Şaban; Karaoğlu, Hakan; Çiçek, Ayşegül Çopur (Korean Soc Laboratory Medicine, 2015)Background: the emergence of carbapenem-resistant Klebsiella pneumoniae poses a serious problem to antibiotic management. We investigated the beta-lactamases in a group of carbapenem-resistant K. pneumoniae clinical isolates ... -
İntraselüler Anoxybacillus amylolyticus ksilanaz’ının klonlanması, saflaştırılması ve karakterizasyonu
Karaoğlu, Hakan; Akpınar, Züleyha (2023)Ksilanazlar [endo-1,4-β-D-ksilanaz (E.C. 3.2.1.8)] ksilanın çevre dostu bir yöntemle işlenmesine ihtiyaç duyulan endüstriyel birçok uygulamada kullanılan son derece önemli enzimlerdir. Bu çalışmada Anoxybacillus amyloliticus ... -
Magnesite enrichment with pseudomonas oryzihabitans isolated from magnesite ore
Karaoğlu, Hakan; Yanmış, Derya; Gürkök, Sümeyra (Taylor & Francis Inc, 2016)Magnesite is a primary source of magnesium and its compounds. the major problem in its practical use are the impurities such as silicon, iron and calcium carbonate. Some magnesite ores in Turkey cannot be used due to a ... -
Modeling and evaluation of the sucrose-degrading activity of recombinantly produced oligo-1,6-glucosidase from A. gonensis
Karaoğlu, Hakan; Balta, Zeynep Dengiz (Taylor & Francis Ltd., 2023)Fructose is the most preferred sugar to provide benefits for sweetening and health. As many industrial enzymes are used to produce High Fructose Syrup (HFS), it is vital to explore alternative enzymes for fructose production. ... -
Molecular analysis of the genus Anoxybacillus based on sequence similarity of the genes recN, flaA, and ftsY
Çolak, Dilşat Nigar; İnan, Kadriye; Karaoğlu, Hakan; Çanakcı, Sabriye; Beldüz, Ali Osman (Kluwer Academic Publishers, 2012)Genome predictions based on selected genes would be a very welcome approach for taxonomic studies. We analyzed three genes, recN, flaA, and ftsY, for determining if these genes are useful tools for systematic analyses in ...
