Investigation of Gpx1 gene expression, serum Gpx1 and selenium levels on colorectal cancer

Abstract

Objective: Colorectal cancer is one of the first lines when examined the death cause of cancer. It is well known that family history, and racial and ethnic background and daily life are related with colorectal cancer and its treatment options are surgery, chemotherapeutic agents, and radiotherapy. One of the factors that play a critical role in the development of colorectal cancer is the oxidative stress. In this research, we aimed to investigate the association of level of glutathione peroxidase 1 (GPX1) and selenium element with colon cancer. Methods: In this study, we aimed to determine expression levels of GPX1 genes and selenium that maintain protection against oxidative stress, with 35 colon cancer patients and their normal and tumor tissues associated with clinical and prognostic aspects by using qRT-PCR and atomic absorption methods. Results: The results of our study showed that GPX1 gene expression were found to be statistically significantly different (two-fold greater in normal tissue; p < 0.05) between normal and tumor tissue. Although there was a positive correlation between serum GPX1 and selenium levels and increase in expression of GPX1 gene and serum GPX1 levels, there was no statistical significance (p>0,05). Conclusion: Colorectal cancer, that many factors are involved in its etiology, we have found that our preliminary studies results might show the potential role of association between GPX1 gene expressions and selenium levels. For this reason, it has been suggested that the subject should be supported by a large-scale group of patients.