Examination of the effects of thiamine and thiamine pyrophosphate on doxorubicin-induced experimental cardiotoxicity
Citation
Polat, B., Suleyman, H., Sener, E., & Akcay, F. (2015). Examination of the effects of thiamine and thiamine pyrophosphate on Doxorubicin-induced experimental cardiotoxicity. Journal of cardiovascular pharmacology and therapeutics, 20(2), 221–229. https://doi.org/10.1177/1074248414552901Abstract
Background and Purpose: To investigate the effect of thiamine and thiamine pyrophosphate on doxorubicin-induced cardiotoxicity biochemically and histopathologically and to examine whether doxorubicin cardiotoxicity is related to the conversion of thiamine into thiamine pyrophosphate and inhibition of thiamine pyrophosphokinase (TPK) enzyme. Experimental Approach: A total of 48 Albino Wistar male rats were used. Rats were divided into groups as thiamine + doxorubicin (TIA + DOX), thiamine pyrophosphate + doxorubicin (TPP + DOX), DOX, and healthy (HEA) groups. One hour after the administration of thiamine and TPP in 25 mg/kg doses, 5 mg/kg doxorubicin were injected to all groups except HEA group during 7 days. Then, the samples were collected for biochemical (glutathione [GSH], malondialdehyde [MDA], DNA damage, creatine kinase (CK), CK-MB, and troponine I [TP-I]), molecular (TPK), and histopathological examinations. Key Results: Oxidant parameters (MDA and DNA damage) decreased and antioxidant parameter (GSH) increased in TPP + DOX group. in addition, levels of CK, CK-MB, and TP-I were low in the TPP + DOX group and high in the TIA + DOX and DOX groups. Cardiac tissue was protected in TPP + DOX group, and no protective effect was observed in TIA + DOX and DOX groups. Messenger RNA expression of TPK was decreased in DOX and TIA + DOX groups. Conclusion and Implications: the cardiotoxic effect of doxorubicin originated from the inhibition of TPK enzyme resulting in insufficient production of thiamine pyrophosphate.
Source
Journal of Cardiovascular Pharmacology and TherapeuticsVolume
20Issue
2Collections
Related items
Showing items related by title, author, creator and subject.
-
Damage induced by paracetamol compared with N-acetylcysteine
Kısaoğlu, Abdullah; Özoğul, Bünyamin; Turan, Mehmet İbrahim; Yılmaz, İsmail; Demiryılmaz, İsmail; Atamanalp, Sabri Selçuk; Bakan, Ebubekir; Süleyman, Halis (Lippincott Williams & Wilkins, 2014)Background: This study investigated the effect of thiamine pyrophosphate (TPP) on oxidative liver damage induced in rats with high-dose paracetamol. Methods: Rats for this experiment were divided into the following groups: ... -
A comparative investigation of biochemical and histopathological effects of thiamine and thiamine pyrophosphate on ischemia-reperfusion induced oxidative damage in rat ovarian tissue
Demiryılmaz, İsmail; Şener, Ebru; Çetin, Nihal; Altuner, Durdu; Akçay, Fatih; Süleyman, Halis (Pharmaceutical Soc Korea, 2013)In this study, the biochemical and histopathological effects of thiamine and thiamine pyrophosphate on ischemia-reperfusion induced oxidative damage in rat ovarian tissue were investigated. Animals were divided into four ... -
Effects of thiamine and thiamine pyrophosphate on epileptic episode model established with caffeine in rats
Turan, Mehmet İbrahim; Tan, Hüseyin; Çetin, Nihal; Süleyman, Halis; Çayır, Atilla (Elsevier, 2014)This study examines the effect of thiamine (TH) and thiamine pyrophosphate (TPP) on epileptic episode model induced in rats with caffeine. Animals were divided into groups and given TH or TPP at doses of 10, 30 or 50 mg/kg ...