Antioxidative status and lipid peroxidation in kidney tissue of rats fed with vitamin B6-deficient diet
Citation
Keles, M., Al, B., Gumustekin, K., Demircan, B., Ozbey, I., Akyuz, M., Yilmaz, A., Demir, E., Uyanik, A., Ziypak, T., & Taysi, S. (2010). Antioxidative status and lipid peroxidation in kidney tissue of rats fed with vitamin B(6)-deficient diet. Renal failure, 32(5), 618–622. https://doi.org/10.3109/0886022X.2010.481737Abstract
Introduction: The aim of this study was to evaluate lipid peroxidation (LP) and free radical scavenging enzyme activities in kidney tissue of vitamin B6-deficient rats. Material and Methods: The rats were divided into control and vitamin B6-deficient groups. After 4 weeks of feeding, animals in all groups were anesthetized by thiopental sodium (50 mgkg). Thoraces were opened, 2 mL blood samples were taken from aortas, then the rats were killed by cervical dislocation, and kidney tissues were removed. Biochemical measurements in kidney tissue were carried out using a spectrophotometer. Results: Total superoxide scavenger activity (TSSA), nonenzymatic superoxide scavenger activity (NSSA), superoxide dismutase (SOD) activities, and antioxidant potential (AOP) values in the vitamin B6-deficient group were significantly lower than those of the control group, whereas glutathione peroxidase (GSH-Px), glutathione reductase (GRD), glutathione-S-transferase (GST) activities, and malondialdehyde (MDA) level were significantly higher than those of the control group (p < 0.05). Discussion: The results show that vitamin B6 deficiency causes an attenuation in antioxidant defense system and an increase in oxidative stress in kidney tissue of rats. © Informa UK, Ltd.
